Coding

Part:BBa_K1197016:Design

Designed by: Alişan Kayabölen   Group: iGEM13_METU_Turkey   (2013-10-02)


fCPR with SacB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1871


Design Notes

SacB sequence was added to upstream of fCPR sequence to create a fusion protein which secrete outside of the cell.


Source

The source of fCPR is Musca domestica genome, and SacB comes from B. subtilis.

References